TrypTag is a project aiming to determine where every trypanosome protein localises within the cell (Dean et al., 2016).
We will tag every protein gene in the genome (excluding VSGs) with mNeonGreen (Shaner et al., 2013) and determine the localisation by microscopy. Wherever possible, we will determine the localisation of the protein when tagged at both the N and C terminus.
We are using long-primer PCR (Dean et al., 2015) to make the tagging amplicons and transfect the PCR product into trypanosomes using 96 well electroporation plates (Dyer et al., 2016). The full workflow is described in Dean et al., 2016.
The cell lines are not kept after imaging as it is extremely rapid to remake the cell line.
You can contact us at the following email address: firstname.lastname@example.org
TrypTag.org contains data from a major and costly project with the aim of providing the cellular localisation of every protein encoded in the trypanosome genome. The project emerged from a number of years development of the technology in the Gull Lab by Richard Wheeler, Jack Sunter and Sam Dean. Our intention is to make the data is available to the community as it is generated. Data collection is expected to be completed in 2019, after which we will provide a definitive summary of the project by publishing papers describing and analysing the full data set. Until the project is complete, data usage terms are based on the Fort Lauderdale agreement, an agreement reached for pre-publication usage of genome data.
Data on TrypTag.org is available immediately and free to use with acknowledgement. However until the project is complete:
To accept these terms and view localisation images and data please click here.
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